| Overview |
Print Version
|
| Product Name |
Anti-Epidermal Growth Factor Receptor (EGFR) Antibody |
| Description |
EGFR is a transmembrane
glycoprotein that is a member of a family of protein
tyrosine kinases crucial in maintaining a normal
balance in cell growth and development. Growth
factor receptors are involved not only in promoting the
proliferation of normal cells but also in the aberrant
growth of many types of human tumors. For example,
the epidermal growth factor receptor (EGFR) is
mutated and/or overexpressed in many common solid
human squamous cell carcinomas including breast,
brain, bladder, lung, gastric, head & neck, esophagus,
cervix, vulva, ovary, and endometrium. Overexpression
of the EGFR gene occurs in carcinomas
with and without gene amplification. EGFR and erbB-
2 are particularly important in breast cancer because
increased production or activation has been
associated with poor prognosis. EGFR belongs to a
family of growth factor receptors, which also includes
ErbB-2/HER-2/neu, ErbB-3/HER-3/neu and ErbB-
4/HER-4neu. EGFR can heterodimerize with each of
the members of this family. |
| Subtype |
polyclonal |
| Application |
WB, IHC, ELISA |
| Raised In |
rabbit |
| Specificity |
This antiserum is directed against human epidermal growth factor receptor (EGFR) and is useful in
determining its presence in ELISA and western blotting experiments. This antibody can detect EGFR from human, mouse and
rat sources. Reactivity of this antibody with EGFR from other species is unknown. |
| Immunogen |
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a
synthetic peptide corresponding to amino acids 1189-1199 of human EGFR protein. |
| Guarantee |
abm guarantees that all our Anti-Epidermal Growth Factor Receptor (EGFR) Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Properties |
|
| Formulation |
Liquid |
| Quantity |
100 μg |
| Clone |
Polyclonal |
| Storage |
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Storage Buffer |
PBS, pH 7.4 with 0.05% sodium azide. |
| Concentration |
1µg/ul |
| Purity |
Affinity Purified |
| Isotype |
IgG |
| Species Reactivity |
human, mouse, rat |
| Anti-Epidermal Growth Factor Receptor (EGFR) Antibody Images |
|
| Specific References |
|
Y052118 has been referenced in 1 publication.
-
Sartor, C.I. et al., (1997) The Role of EGF Receptor and STAT-3 Activation in Autonomous Proliferation of SUM-102PT Human Breast Cancer
Cells. Cancer Research 57:978.
Scambia G, et al., (1993) Expression of ras oncogene p21 protein in normal and neoplastic ovarian tissues: Correlation with histopathological
features and receptors for estrogen, progesterone, and the epidermal growth factor. Am J Obstet gynecol 168:71-78.
Henzen-Logmans, S.C., et al., (1993) Occurrence of epidermal growth factor receeptors in benign and malignant ovarian tumors and normal
ovarian tissues: an immunohistochemical study. J Can Res Clin Oncol 188:303-307.
Sibilia, M., A.B. et al., (2000). The EGF Receptor Provides an Essential Survival Signal for SOS-Dependent Skin Tumor Development. Cell
102, 211-220.
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