Zebrafish Testicular Feeder Cells (ZtA6-4) T2603|产品详情|进口试剂采购网

您好，欢迎来到拜力生物网站！请登录注册

我的订单 | 联系我们 | 在线客服QQ：3012681483 | 客服电话：400-968-7988 | 工作时间：08:30-17:30

主营产品： ELISA试剂盒、抗体、补体血清、兔补体、干扰素、小鼠测温仪、进口试剂、Quidel代理、Swant代理、PBL Assay Science代理

网站首页产品中心代理品牌新闻资讯资源中心关于我们

所在位置：首页 > 产品中心 > abmgood > abmgood产品

Zebrafish Testicular Feeder Cells (ZtA6-4)

订购信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Zebrafish Testicular Feeder Cells (ZtA6-4)

品牌：Abmgood

货号：T2603

规格：

货期：

应用：

咨询客服

立即购买加入购物车

Zebrafish Testicular Feeder Cells (ZtA6-4)

商品详情参考文献相关资料

	Print Version
Safety	BioSafety Level II
Organism	Albino-type (alb-1/alb-1) zebrafish (Danio rerio)
Source Organ	Testis
Growth Properties	Adherent
Morphology	Epithelial and fibroblast-like
Applications	For Research Use Only.
Picture
Picture
Picture
Description	ZtA6 cells are derived from spontaneous tumor-like hypertropheied testis isolated from albino-type (alb-1/alb-1) zebrafish. Clones were analyzed for the expression of the Sertoli cell marker (Sox9a), the germ cell marker (Vas), and the Wilm’s tumor suppressor marker (WT1). 12 clones were derived and have distinctive properties and are available at abm. ZtA6-4 cells are clone 4 which has high phagocytic ability compared to the other clones derived from the ZtA6 cell line. The expression of Sox9a and Vas were observed, but WT1 expression was absent. It is able to support the differentiation of germ cells into functional sperm for in vitro fertilization, thus is a suitable tool for research in in vitro fertilization and vertebrate spermatogenesis.
Quality Control	1) Phagocytic activity was analyzed via the ability to internalize polystyrene beads; 2) RT-PCR was used to assess the presence or absence of WT1, Vas, and Sox9a markers; 3) Functionality test was performed to determine the ability to support male germ cells when co-cultured as feeder cells.
Propagation	Grow cells in T25 gelatin-coated flasks (TM063) with the following conditions. The base medium for this cell line is L-15 medium (ThermoFisher Scientific). To make the completed growth medium, add the following components to the base medium at the following final concentrations: 5 IU/ml human chorionic gonadotropin (Sigma), 2 IU/ml pregnant mare’s serum gonadotropin (Sigma), 0.2 mg/ml L-arginine (Gibco BRL), 0.02 mg/ml L-aspartic acid (Gibco BRL), 0.015 mg/ml L-histidine (Gibco BRL), 0.0725 mg/ml L-lysine HCl (Gibco BRL), 0.02 mg/ml L-proline (Gibco BRL), 0.5% bovine serum albumin (5% w/v stock solution, BSA fraction V, Sigma), 1% Hepes (Sigma), 3% fetal bovine serum (TM999), and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 100%, Temperature: 28.0°C. To prepare as feeder cells: Treat with 10 µg/ml mitomycin C in L-15 for 5 hours before plating for use as feeder cells for further experimentation.
Storage	-180°C
Shipping	On Dry Ice
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.