Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Lac+ colones grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.
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