Ready-to-use fluorometric substrate for caspases that recognize the amino acid sequence VDVAD. Caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate VDVAD-AFC at Ex/Em = 400/505 nm using a fluorometer or a fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat.# 1067-100), 2X Reaction Buffer (Cat.# 1068-20), DTT (Cat.# 1201-1), and other reagents used for caspase activity assays are also available separately.

Additional Information

Synonyms	Ac-VDVAD-AFC, Caspase-2 Substrate, Fluorogenic
Product #	C-2236
Chemical Name	Ac-VDVAD-AFC, Caspase-2 Substrate, Fluorogenic
Formula	C33H41F3N6O12
MW	770.7
Appearance	Liquid
Purity	98% by HPLC
Storage Temp	-20°C
Use	A fluorogenic substrate for caspase-2

Additional Information

MDL Number	MFCD04037014
ChemACX	X1340851-6
InChI	InChI=1S/C33H41F3N6O12/c1-13(2)26(38-16(6)43)32(53)41-21(12-24(46)47)30(51)42-27(14(3)4)31(52)37-15(5)28(49)40-20(11-23(44)45)29(50)39-17-7-8-18-19(33(34,35)36)10-25(48)54-22(18)9-17/h7-10,13-15,20-21,26-27H,11-12H2,1-6H3,(H,37,52)(H,38,43)(H,39,50)(H,40,49)(H,41,53)(H,42,51)(H,44,45)(H,46,47)/t15-,20-,21-,26-,27-/m0/s1
SMILES	C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)Nc1ccc2c(cc(=O)oc2c1)C(F)(F)F)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](C(C)C)NC(=O)C
Peptide Sequence	AC-VAL-ASP-VAL-ALA-ASP-AFC