Ready-to-use colorimetric substrate for caspase-9/Mch6 and related caspases that recognize the amino acid sequence LEHD. Caspase-9 and related caspase activity can be quantified by spectrophotometric detection of free pNA (l= 400 nm) after cleavage from the peptide substrate LEHD-pNA, using a spectrophotometer or multi-well plate reader.

Additional Information

Synonyms	Ac-LEHD-pNA, Caspase-9 Substrate, Fluorogenic
Product #	C-2255
Chemical Name	Ac-LEHD-pNA, Caspase-9 Substrate, Fluorogenic
Formula	C29H38N8O11
MW	674.7
Appearance	Liquid
Purity	98% by HPLC
Storage Temp	-20°C
Use	Colorimetric substrate for caspase-9/Mch6 and related caspases that recognize the amino acid sequence LEHD

Additional Information

MDL Number	MFCD02094704
ChemACX	X1206348-4
InChI	InChI=1S/C29H38N8O11/c1-15(2)10-21(32-16(3)38)28(45)34-20(8-9-24(39)40)26(43)35-22(11-18-13-30-14-31-18)29(46)36-23(12-25(41)42)27(44)33-17-4-6-19(7-5-17)37(47)48/h4-7,13-15,20-23H,8-12H2,1-3H3,(H,30,31)(H,32,38)(H,33,44)(H,34,45)(H,35,43)(H,36,46)(H,39,40)(H,41,42)
SMILES	CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(CC1=CN=CN1)C(=O)NC(CC(=O)O)C(=O)NC2=CC=C(C=C2)[N+](=O)[O-])NC(=O)C
Peptide Sequence	AC-LEU-GLU-HIS-ASP-PNA