Ready-to-use colorimetric substrate for CPP32/caspase-3 and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by spectrophotometric detection of free pNA (l = 400 nm) after cleavage from the peptide substrate DEVD-pNA, using a spectrophotometer or multi-well plate reader. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amounts of caspase assays.

Additional Information

Synonyms	Ac-DEVD-pNA, Caspase-3 Substrate, Colorimetric
Product #	C-2240
Chemical Name	Ac-DEVD-pNA, Caspase-3 Substrate, Colorimetric
Formula	C26H34N6O13
MW	638.58
Appearance	Liquid
Purity	98% by HPLC
Storage Temp	-20°C
Use	A colorimetric substrate for caspase-3 (CPP32) and related cysteine proteases

Additional Information

MDL Number	MFCD00792707
ChemACX	X1218447-3
InChI	InChI=1S/C26H34N6O13/c1-12(2)22(26(43)30-18(11-21(38)39)24(41)28-14-4-6-15(7-5-14)32(44)45)31-23(40)16(8-9-19(34)35)29-25(42)17(10-20(36)37)27-13(3)33/h4-7,12,16-18,22H,8-11H2,1-H3,(H,27,33)(H,28,41)(H,29,42)(H,30,43)(H,31,40)(H,34,35)(H,36,37)(H,38,39)
SMILES	CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC=C(C=C1)[N+](=O)[O-])NC(=O)C(CCC(=O)O)NC(=O)C(CC(=O)O)NC(=O)C
Peptide Sequence	AC-ASP-GLU-VAL-ASP-PNA