Applications: Thiophilic affinity purification is based on the ability of some proteins, particularly immunoglobulins, to bind to this immobilized ligand. The term "Thiophilic" refers to an affinity for sulfone groups that lie in close proximity to thioether groups. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Thiophilic 4B-CL is useful in purification of proteins, especially immunoglobulin G. Thiophilic 4B-CL allows purification of immunoglobulins from all animal species under mild conditions (pH 8.0, low salt concentration, elution at physiological pH).

Technical specifications:

• Active group: 2-Mercaptopyridine
• Matrix: 4B-CL (highly crosslinked agarose, 4%)
• Particle size range: 52 – 165 μm
• Matrix activation: Divinyl sulfone
• Matrix attachment: Thiol
• Matrix spacer: 5 atoms
• Active group density: 3 mg / ml drained gel
• Binding capacity: 30 – 45 mg human IgG / ml drained gel
• pH stability: 3 – 11 (long term), 2 – 13 (cleaning)

Additional Information

Additional Information