Ready-to-use fluorometric substrate for caspase-4 and related caspases that recognize the amino acid sequence LEVD. Caspase-4 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

Additional Information

Synonyms	Ac-LEVD-AFC, Caspase-4 Substrate, Fluorogenic
Product #	C-2242
Chemical Name	Ac-LEVD-AFC, Caspase-4 Substrate, Fluorogenic
Formula	C32H40F3N5O11
MW	727.5
Appearance	Liquid
Purity	99% by HPLC
Storage Temp	-20°C
Use	Fluorometric substrate for caspase-4 and related caspases that recognize the amino acid sequence LEVD

Additional Information

MDL Number	MFCD01862605
ChemACX	X1340772-0
InChI	InChI=1S/C32H40F3N5O11/c1-14(2)10-21(36-16(5)41)30(49)38-20(8-9-24(42)43)28(47)40-27(15(3)4)31(50)39-22(13-25(44)45)29(48)37-17-6-7-18-19(32(33,34)35)12-26(46)51-23(18)11-17/h6-7,11-12,14-15,20-22,27H,8-10,13H2,1-5H3,(H,36,41)(H,37,48)(H,38,49)(H,39,50)(H,40,47)(H,42,43)(H,44,45)
SMILES	CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C
Peptide Sequence	AC-LEU-GLU-VAL-ASP-AFC