Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 M) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

Additional Information

Synonyms	Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic
Product #	C-2239
Chemical Name	Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic
Formula	C30H33F3N5O13
MW	729
Appearance	Liquid
Purity	98% by HPLC
Storage Temp	-20°C
Use	A fluorogenic substrate for caspase-3 and related cysteine proteases

Additional Information

MDL Number	MFCD01310970
ChemACX	X1206324-4
InChI	InChI=1S/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)/t17-,18-,19-,25-/m0/s1
SMILES	CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CCC(=O)O)NC(=O)C(CC(=O)O)NC(=O)C
Peptide Sequence	AC-ASP-GLU-VAL-ASP-7-AFC