Applications:

Protein A binds specifically to F_c regions of many mammalian immunoglobulins. Protein A-conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids. Protein A- 4B-CL is used for purification and/or fractionation of IgG subclasses. It is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. This is also commonly used to capture immune complexes generated in immunoprecipitation (IP) experiments.

Technical Specifications

• Ligand: recombinant Protein A from E. coli
• Matrix: 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 – 165 µm
• Matrix activation: Cyanogen bromide
• Molecular weight range: 6 x 10⁴ -  2 x 10⁷
• Ligand density:  ~3 mg Protein A / ml drained gel
• Binding capacity: ~ 20 mg human IgG / ml drained gel
• pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
• Storage: 2 – 8 °C.
• Supplied as 50% slurry in PBS with sodium azide

Additional Information

Additional Information