Agglutination activity is expressed in mg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 µg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of trypsinized human blood group O erythrocytes after 1 hr at 25 °C.

ECA is not blood group specific, but has an affinity for D-galactose and D-galactosides. It is reported to be mitogenic for human peripheral blood T lymphocytes.

Purified by affinity chromatography.

Technical Specifications:

• Ligand Source: Erythrina cristagalli
• Molecular Weight: 54 kDa
• Carbohydrate Specificity: Galβ1,4GlcNAc
• Inhibiting/Eluting Sugar: Lactose
• Carbohydrate for Elution: 0.2M Lactose
• Blood Group Specificity: A (-SA)
• Form: Lyophilized
• Isoelectric (pI): 6.3-6.5

Additional Information

Additional Information