Applications:

Ni²⁺-chelated 4B-CL is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that separates proteins via their differences in affinity to nickel metal ions. Ni²⁺-chelated 4B-CL is ideal for purification of recombinant proteins containing (His)₆ fusion tag.

Technical Specifications:

• Chelating group: Nickel
• Matrix: 4B-CL (crosslinked agarose beads, 4%)
• Matrix activation: Epoxy
• Particle size range: 52 – 165 µm
• Metal ion capacity: >20µmol Ni²⁺ / ml drained gel
• Binding capacity: ~5 – 8 mg (His)₆-tagged protein / ml medium
• pH stability: 2 – 11
• Chemical stability: For one week at 40 °C: 0.01M HCl, 0.1M NaOH

        For 12 h: 1M NaOH, 70% acetic acid.

       For 30 min tested: 30% 2-propanol.

        For 1 h tested: 2% SDS 

• Compatibility during use: Stable in all commonly used buffers, reducing agents, denaturants, and detergents
• Supplied as suspension in 20% ethanol

Additional Information

Additional Information