Applications:

NAD covalently bound to has a high affinity for NAD-dependent enzymes and dehydrogenases. NAD- 4B-CL has been used to purify Vibrio cholerae toxin, diptheria toxin and other NAD binding proteins.

Technical Specifications:

• Ligand: NAD
• Matrix: 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 – 165 µm
• Matrix activation: Cyanogen bromide
• Matrix attachment: N-6 
• Ligand density: 2 µmol NAD / ml drained gel
• pH stability: 4 – 10
• Supplied as lyophilized powder stabilized with lactose
• Swelling: 1 g swells to approximately 5 ml

Additional Information

Additional Information