Applications: Reduced glutathione is covalently linked to 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated 4 % crosslinked agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity of 5 mg or more of Glutathione-S-transferase (GST) per ml of wet gel.. Bound GST –fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function.

Technical specifications:

• Ligand: Reduced glutathione (GSH)
• Matrix: 4B (agarose beads, 4%)
• Particle size range: 52 – 165 µm
• Molecular weight range: 6 x 10⁴ – 2 x 10⁷
• Matrix activation: Epoxy
• Matrix spacer: 12 atoms
• Ligand density: 5 – 10 mg GST / ml drained gel
• Binding capacity: 5 – 10 mg recombinant GST-tagged protein / ml drained gel
• pH stability: 4 – 13
• Storage: 4 °C – 30 °C 
• Supplied as suspension in 20% ethanol

Additional Information

Additional Information