MES is a zwitterionic N-substituted aminosulfonic acid, active in the pH range 6-8 with a pKa of 6.10 at 25°C. With a pKa value near physiological pH, it is not absorbed through cell membranes, and it is essentially transparent to UV light. MES is used as a running buffer for resolving very small proteins on bis-tris gels. Also used in cation-exchange chromatography and as a biological buffer in plant cell cultures.
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