FH1 is a potent and selective small molecule that enhances hepatocyte functions and promotes the differentiation and maturation of iPSC-derived hepatocytes toward a more mature phenotype in vitro. FH1 was identified by a high-throughput, cell-based screening using primary human hepatocytes. It was added to the basal medium  with or without OSM on day 21 after the initiation of differentiation of iPSCs and acted over a period of 9 day to promote more pronounced hepatocyte morphologies, including polygonal cell shapes, visible nuclei and more noticeable bile canaliculi between hepatocytes.

How to Use:

• In vitro:  FH1 was suggested to be supplemented into the culture medium on day 21 at a concentration of 15-20 μM to enhance hepatocyte functions in vitro over period of 9 days.
• In vivo: n/a

Reference:

• 1. Shan J, et al. Identification of small molecules for human hepatocyte expansion and iPS differentiation. (2013) Nat Chem Biol. 9(8):514-20.