Rapamycin is a potent and selective mTOR inhibitor. Rapamycin, in complex with the cytosolic protein FK-binding protein 12 (FKBP12), inhibits mTOR Complex 1 (mTORC1) by binding to the FRB domain of mTor. Given the central roles mTor plays, Rapamycin showed diverse activities, including anti-proliferative effects in cancer cells, immunosuppressive effect, lifespan extension in mice, improved cognition in the mouse model of autism and Alzheimer, and inducing autophagy.

How to Use:

• In vitro: Rapamycin is usually used at 1 µM final concentration in vitro and in cellular assays.
• In vivo: Rapamycin could be orally administrated to mice at 5-20 mg/kg once per day.

Reference:

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