Biological Activity:

ISX9 is a neurogenic modulator. It mediates neuroD reporter gene induction via activation of Ca2+ influx, and increases expression of neurogenic differentiation 1 (NeuroD1) transcription factor. It induces neuronal differentiation in human cortical neuronal cells (HCN), ***** mouse whole brain (MWB) and subventricular zone (SVZ) progenitors. ISX9 is also shown to induce cardiomyogenic differentiation of Notch-activated epicardium-derived cells in vitro.

How to Use:

In vitro: ISX9 was used at 10-20 µM final concentration in vitro and in cellular assays.
In vivo:

Reference:

• 1. Schneider JW, et al. Small-molecule activation of neuronal cell fate. (2008) Nat Chem Biol. 4(7):408-10. 
• 2. Dioum EM, et al. A small molecule differentiation inducer increases insulin production by pancreatic β cells. (2011) Proc Natl Acad Sci USA. 108(51):20713-8. 
• 3. Zhang L, et al. Small-molecule blocks malignant astrocyte proliferation and induces neuronal gene expression. (2011) Differentiation. 81(4):233-42. 
• 4. Russell JL, et al. Targeting native ***** heart progenitors with cardiogenic small molecules. (2012) ACS Chem Biol. 7(6):1067-76. 
• 5. Li X, et al. Small-Molecule-Driven Direct Reprogramming of Mouse Fibroblasts into Functional Neurons. (2015) Cell Stem Cell. 17(2):195-203.