AK-7 is a cell- and brain-permeable, selective sirtuin SIRT2 inhibitor (IC₅₀ ~15.5 μM), displays no effect on SIRT1 or SIRT3. It diminishes neuronal cell death induced by mutant huntingtin fragment. It also down-regulates cholesterol biosynthetic gene expression and reduces total cholesterol levels in neurons in vivo. In two genetic mouse models of Huntington’s disease, AK-7 treatment resulted in improved motor function, extended survival, and reduced brain atrophy. AK-7 is a good chemical probe to study SIRT2’s roles in metabolic diseases, cancer, age-related disorders, and neurodegenerative diseases. 

How to Use:

In vitro: AK-7 was used at 10-25 µM in vitro and in cellular assays.

In vivo: AK-7 was administered by intraperitoneal injection to mice at 15-30 mg/kg/dose twice daily (formulation: 1.5 mg/mL in 25% Cremophor/10% DMSO in water).

Reference:

1. 1.  Taylor DM, et al. A brain-permeable small molecule reduces neuronal cholesterol by inhibiting activity of sirtuin 2 deacetylase. (2011) ACS Chem Biol. 6(6):540-6.
2. 2.  Chopra V, et al. The sirtuin 2 inhibitor AK-7 is neuroprotective in Huntington's disease mouse models. (2012) Cell Rep. 2(6):1492-7. 

Products are for research use only. Not for human use.