成纤维细胞是来源于中胚层的间充质细胞。乳腺的细胞外基质大部分为乳腺成纤维细胞（MF）所分泌。更重要的是这些细胞外基质通过影响基因表达，调节乳腺上皮细胞的增殖和分化。另外，有研究表明乳腺成纤维细胞能招募巨噬细胞的免疫监视，这与细胞外基质产生、新生血管生成等共同作用，形成乳腺微环境，促进肿瘤细胞的发展。这些结果表明，MF能够刺激乳腺癌的发展和侵袭。
Sciencell研究实验室的MF是从人乳腺分离得到的，原代冻存，冰冻运输。每管细胞密度*过5×10^5/ml。细胞特性经纤维粘连蛋白CD90、vimentin免疫荧光鉴定。本细胞经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。如采用ScienCell 实验室特制的培养基，可保证此细胞15倍增增殖。
推荐培养基：成纤维细胞培养基 (FM, Cat. #2301)
Question:
Dear Sir, I would like to ask few questions. 1. What is the maximum passage number I can subculture the cells to, such that there is no significant genotypic or phenotypic change compared to cells at passage 0 as I received initially? 2. Is there a possibility to mix the cells from 3 donors into one vial with total cells of let say about 1 million cells/vial? Thank you very much for your time and attention. Would like to hear from you. Best regards, Aurino
Answers:
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As stated in the product sheet, Human Mammary Fibroblasts are guaranteed to expand for 15 population doublings under the conditions provided. Please note that population doubling is not the same as passage number – for help, please see our Frequently Asked Questions section under Technical Resources. We do not have quantitative data measuring phenotypic or genetic changes over passage number. Unfortunately, each lot of cells is derived from one donor, so we cannot provide one vial of cells pooled from multiple donors. However, customers can request vials from different donors when purchasing more than one vial.
