肝脏星形细胞是小叶内结缔组织细胞，呈现肌成纤维细胞样或脂肪细胞形态。他们参与肝脏细胞外基质的稳态，修复，再生，纤维化以有控制视黄醇的代谢，存储和释放。肝脏受伤后，肝脏星形细胞转化成肌成纤维细胞样，并且是纤维化的肝脏中 I 型胶原的主要来源。除了以上特征，已发现在疾病状态和肝内门静脉高压的发病过程中，肝星状细胞通过细胞收缩调节肝脏微循环[1,2]。星形细胞的增殖、迁移和趋化因子的表达参与了肝脏炎症和纤维化的发病过程。因此，星形细胞激活的分子调控在将来有助于治疗肝纤维化，并且能减少慢性肝病患者的发病率和死亡率。
Sciencell研究实验室的人肝星形细胞是从人肝脏组织中分离得到的，原代冻存，冰冻运输。每管细胞密度*过5×10^5/ml。人脐静脉平滑肌细胞经desmin 和α-actin免疫荧光鉴定。本细胞经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。如采用ScienCell 实验室特制的培养基，可保证此细胞15倍增增殖。? ?
推荐培养基：星形细胞培养基?(SteCM, Cat. #5301)
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货号
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5300
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产地
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美国
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缩写
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HHSteC
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规格
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5 x 10^5 /1ml
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用途
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科研
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储存
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液氮
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运输
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干冰
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1.) Wagner I,?Materne EM,?Brincker S,?S??bier U,?Fr?drich C,?Busek M,?Sonntag F,?Sakharov DA,?Trushkin EV,?Tonevitsky AG,?Lauster R,?Marx U. (2013) "A dynamic multi-organ-chip for long-term cultivation and substance testing proven by 3D human liver and skin tissue co-culture."?Lab Chip. doi: 10.1039/c3lc50234a
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5.) Mart?n-V?lchez S, Sanz-Cameno P, Rodr?guez-Mu?oz Y, Majano PL, Molina-Jim?nez F, L?pez-Cabrera M, Moreno-Otero R, Lara-Pezzi E. (2008) "The hepatitis B virus X protein induces paracrine activation of human hepatic stellate cells."?Hepatology. 47: 1872-83.
6.) Semela D, Das A, Langer D, Kang N, Leof E, Shah V. (2008) "Platelet-derived growth factor signaling through ephrin-b2 regulates hepatic vascular structure and function."Gastroenterology. 135: 671-9.
7.) Shimamura T, Fujisawa T, Husain SR, Kioi M, Nakajima A, Puri RK. (2008) "Novel role of IL-13 in fibrosis induced by nonalcoholic steatohepatitis and its amelioration by IL-13R-directed cytotoxin in a rat model."?J Immunol. 181: 4656-65.
8.) Che J, Chan ES, Cronstein BN. (2007) "Adenosine A2A receptor occupancy stimulates collagen expression by hepatic stellate cells via pathways involving protein kinase A, Src, and extracellular signal-regulated kinases 1/2 signaling cascade or p38 mitogen-activated protein kinase signaling pathway."?Mol Pharmacol. 72: 1626-36.
9.) Perri RE, Langer DA, Chatterjee S, Gibbons SJ, Gadgil J, Cao S, Farrugia G, Shah VH. (2006) "Defects in cGMP-PKG pathway contribute to impaired NO-dependent responses in hepatic stellate cells upon activation."?Am J Physiol Gastrointest Liver Physiol. 290: G535-42.
10.) Brown KE, Broadhurst KA, Mathahs MM, Brunt EM, Schmidt WN. (2005) "Expression of HSP47, a collagen-specific chaperone, in normal and diseased human liver."?Lab Invest. 85: 789-97.
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