口腔黏膜角化细胞是口腔疾病主要屏障，保护局部细胞免受微生物和化学物质造成的伤害。它们通过不断产生或受刺激后产生的细胞因子，刺激参与促发炎症反应，这意味着它们可能通过炎症过程潜在地参与口腔感染的调控，该过程需要不同的白细胞介素参与，如IL-1?和IL-18。口腔黏膜角化细胞表达多种不同的细胞标志物，该表达受到目标基因转录过程中钙素诱导变化的影响。口腔黏膜角化细胞与特征明显的皮肤角质细胞在结构和功能上有很大的相似性，并且为研究角质细胞的生物学特性、无限增殖过程、恶性转化提供了良好的模型。
人口腔黏膜角化细胞提取于人口腔黏膜，一代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过Cytokeratine-8, -18 and -19免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。

推荐培养基：口腔角质细胞培养基 (OKM, Cat. #2611)

货号	2610
产地	美国
缩写	HOK
规格	5 x 10^5 /1ml
用途	科研
储存	液氮
运输	干冰

1.) Yen YC, Shiah SG, Chu HC, Hsu YM, Hsiao JR, Chang JY, Hugn WC, Liao CT, Cheng AJ, Lu YC, Chen YW. (2014) 'Reciprocal regulation of MicroRNA-99a and insulin-like growth factor I receptor signaling in oral squamous cell carcinoma cells.'?Mol cancer.

2.) Yen YC, Shiah SG, Chu HC, Hsu YM, Hsiao JR, Chang JY, Hugn WC, Liao CT, Cheng AJ, Lu YC, Chen YW. (2014) "Reciprocal regulation of MicroRNA-99a and insulin-like growth factor I receptor signaling in oral squamous cell carcinoma cells."?Mol cancer. 13: 6.

3.) Ji W, Lee C, Chen JY, Cheng Y, Yang S, Chen J, Chen H. (2013) "Areca Nut Extract Induces Pyknotic Necrosis in Serum-Starved Oral Cells via Increasing Reactive Oxygen Species and Inhibiting GSK3: An Implication for Cytopathic Effects in Betel Quid Chewers."?PLoS One. 8: e63295.

4.) Wallet MA, Calderon NL, Alonso TR, Choe CS, Catalfamo DL, Lalane CJ, Naiva KG, Panagakos F, Wallet SM. (2013) ?Triclosan alters antimicrobial and inflammatory responses of epithelial cells.??Oral dis.?19: 296-302.

5.) Talwar S, Balasubramanian S, Sundaramurthy S, House R, Wilusz CJ, Kuppuswamy D, D'Silva N, Gillespie MB, Hill EG, Palanisamy V. (2013) "Overexpression of RNA-binding protein CELF1 prevents apoptosis and destabilizes pro-apoptotic mRNAs in oral cancer cells."?RNA Biol. 10: 277-86.

6.) Lee SS, Tsai CH, Yu CC, Chang YC. (2013) "Elevated Snail Expression Mediates Tumor Progression in Areca Quid Chewing-Associated Oral Squamous Cell Carcinoma via Reactive Oxygen Species."?PLoS One. 8: e67985.

7.) Kim JS, Yu SK, Lee MH, Park MG, Park E, Kim SG, Lee SY, Kim CS, Kim HJ, Chun HS, Chun SW, Kim DK. (2013) 'MicroRNA-205 directly regulates the tumor suppressor, interleukin-24, in human KB oral cancer cells.'

8.) Kim JS, Yu SK, Lee MH, Park MG, Park E, Kim SG, Lee SY, Kim CS, Kim HJ, Chun HS, Chun SW, Kim DK. (2013) "MicroRNA-205 directly regulates the tumor suppressor, interleukin-24, in human KB oral cancer cells."?Mol cells. 35: 17-24.

9.) Russo N, Wang X, Liu M, Banerjee R, Goto M, Scanlon C, Metwally t, Inglehart RC, Tsodikov A, Duffy S, Tubergen EV, Bradford C, Carey T, Wolf G, Chinnaiyan AM, D

10.) Kumar B, Yadav A, Lang JC, Cipolla MJ, Schmitt AC, Arradaza N, Teknos TN, Kumar P. (2012) 'YM155 reverses cisplatin resistance in head and neck cancer by decreasing cytoplasmic survivin levels.'

11.) Kumar B, Yadav A, Lang JC, Cipolla MJ, Schmitt AC, Arradaza N, Teknos TN, Kumar P. (2012) "YM155 reverses cisplatin resistance in head and neck cancer by decreasing cytoplasmic survivin levels."?Mol cancer ther.?11: 1988-98.

12.) Van Tubergen E, Vander Broek R, Lee J, Wolf G, Carey T, Bradford C, Prince M, Kirkwood KL, D'Silva NJ. (2011) "Tristetraprolin regulates interleukin-6, which is correlated with tumor progression in patients with head and neck squamous cell carcinoma."?Cancer. 117: 2677-89.

13.) Jiang L, Liu X, Kolokythas A, Yu J, Wang A, Heidbreder CE, Shi F, Zhou X. (2010) "Downregulation of the Rho GTPase signaling pathway is involved in the microRNA-138-mediated inhibition of cell migration and invasion in tongue squamous cell carcinoma."?Int J Cancer. 127: 505-12.

14.) Mitchell C, Joyce AR, Piper JT, McKallip RJ, Fariss MW. (2010) "Role of oxidative stress and MAPK signaling in reference moist smokeless tobacco-induced HOK-16B cell death."?Toxicol Lett. 195: 23-30.

15.) Phan TC, Ooi J, Goonewardene MS. (2010) "A novel molecule, SLURP-1, enhances the survival of periodontal ligament fibroblasts."?J Periodontal Res. 45: 331-6.?

16.) Rao SK, Pavicevic Z, Du Z, Kim JG, Fan M, Jiao Y, Rosebush M, Samant S, Gu W, Pfeffer LM, Nosrat CA. (2010) "Pro-inflammatory genes as biomarkers and therapeutic targets in oral squamous cell carcinoma."?J Biol Chem. 285: 32512-21.

17.) Dowejko A, Bauer RJ, M?ller-Richter UD, Reichert TE. (2009) "The human homolog of the Drosophila headcase protein slows down cell division of head and neck cancer cells."?Carcinogenesis. 30: 1678-85.

18.) Ghosh A, Park JY, Fenno C, Kapila YL. (2008) "Porphyromonas gingivalis, gamma interferon, and a proapoptotic fibronectin matrix form a synergistic trio that induces c-Jun N-terminal kinase 1-mediated nitric oxide generation and cell death."?Infect Immun. 76: 5514-23.

19.) M?ller-Richter UD, Dowejko A, Zhou W, Reichert TE, Driemel O. (2008) "Different expression of MAGE-A-antigens in foetal and ***** keratinocyte cell lines."?Oral Oncol. 44: 628-33.?

20.) Liang SC, Tan XY, Luxenberg DP, Karim R, Dunussi-Joannopoulos K, Collins M, Fouser LA. (2006) "Interleukin (IL)-22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptides."?J Exp Med. 203: 2271-9.

Question:

I use poly-L-lysine to coat my plate to culture HOK(Human Oral Keratinocytes) cells. I also found out that we have poly-D-lysine. Can I use poly-D-lysine to coat my plate and culture HOK or other primary cells?

Answers:

Thanks for asking! Although we have not tested it, poly-D-lysine should work for these cells.
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