货号	1800
产地	美国
缩写	HA
规格	5 x 10^5 /1ml
用途	科研
储存	液氮
运输	干冰

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1、Question:

Which genes could represent good housekeeping genes for RT-qPCR analysis of these cells? Any suggestions?

Answers:

We recommend β-actin, GAPDH, LDHA, NONO, and PPIH to serve as housekeeping genes for astrocyte qRT-PCR. We also recommend using multiple housekeeping genes for data normalization. In addition, in a qRT-PCR assay, we recommend using Genomic DNA (gDNA) Control (GDC) to detect possible gDNA contamination, and Positive PCR Control (PPC) to test whether samples contain inhibitors or other factors that may negatively affect gene expression results. ScienCell offers GeneQuery? Human Astrocyte Cell Biology qPCR Array Kit for astrocyte qRT-PCR analysis.

2、Question:

WAre this cell lines？

Answers:

They are primary cells, not immortalized cell lines. thanks for your interest

3、Question:

What is the recommended maximum number of passages that the primary human astrocytes can be grown to without losing phenotype? Is there a recommended freezing protocol?

Answers:

Human Astrocytes can be subcultured for 4-5 passages. However, for primary cells, the markers such as GFAP may be lost during each passage, even though they are still astrocytes. Therefore, we recommend that customers use the cells at early passage without expanding or passaging too much. We do not recommend re-freezing the cells for future use. thanks for your interest