雪旺细胞是神经嵴的衍生物，形成周围神经髓磷脂轴突的髓鞘。它们分别环绕在周围神经轴突的轴上，沿着轴突节形成一层髓磷脂髓鞘。雪旺细胞对周围神经的发育，功能和再生起着重要作用。当轴突快死亡时，雪旺细胞环绕其周围帮助消化轴突。留下连续的雪旺细胞形成一个空的管道，新的轴突在管道的末端开始生长。在周围神经中雪旺细胞的数量是受到严格调控的。体外增殖受到诸如PDGF，FGF，神经元和其它多肽类生长因子的刺激。雪旺细胞提供相对简单，明确，易得的哺乳动物模型以用来研究一系列发育问题。了解血旺细胞的生物学特征具有重要的**意义，不仅对于研究神经病变产生的环境和神经的再生，而且细胞或他们的前体可能特别适合用作**神经系统修复的植入物。
ScienceCell实验室的HSC提取自人脊髓神经，原代冻存，冰冻运输。每管细胞密度*过5×105/ml。细胞经S-100,?GFAP?和?CD90免疫荧光鉴定。本细胞经检测不含HSC?HIV-1,?HBV,?HCV,?支原体,?细菌,?酵母菌和真菌。如采用ScienCell?实验室特制的培养基，可保证此细胞10次倍增。? ?
推荐培养基：雪旺细胞培养基(SCM, Cat. #1701)
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货号
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1700
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产地
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美国
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缩写
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HSC
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规格
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5 x 10^5 /1ml
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用途
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科研
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储存
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液氮
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运输
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干冰
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1、Question:
What is the best way you suggest to cryopreserve primary human Schwann cells?
Answers:
We do not recommend that customers re-freeze our cells since primary cells are fragile and they may be damaged during re-freeze and re-thaw process.
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2、Question:
In the product description, these cells were analyzed by immunofluorescence using antibodies against S100, GFAP, and CD90. Would you be able to provide manufacturer/catalog# for those antibodies? Your protocols for cell preparation and immunofluorescence using those antibodies would be kindly appreciated as well? Thank you
Answers:
Posted by Manoj Sharma on Thursday, January 14, 2016 Dear Amish, Here are the antibodies we use for staining #1700 Human Schwann Cells: （1）S-100 beta: Sigma Cat. No. S2532 1:500 dilution （2）GFAP: Sigma Cat. No. G3893 1:1000 dilution And following is the protocol that we use for staining the cells. Immunofluorescence Staining Protocol a. Fixing the slide i. Wash each well with 500 μl PBS making sure to add along the side of the well wall. ii. Add 500 μl 4% PFA (paraformaldehyde) to each well and place at room temperature for 5 minutes. b. Blocking Solution i. Wash each well with 500 μl PBS. ii. The blocking solution consists of 5% NGS (Normal Goat Serum) and 0.1% Triton-X-100 in PBS. iii. The blocking solution can be made in bulk in a 15mL conical tube and then placed in the wells. iv. For every 1 ml of PBS add 50 μl of NGS and 10 μl of 10% Triton-X 100. Add 250 μl per well and incubate at room temperature for 1 hour. c. Primary Antibody i. Prepare the solution for the primary antibody. This consists of 1% NGS and 1% Triton-X 100 in PBS. For every 1 ml of PBS add 10 μl of NGS and 10 μl of Triton-X 100. This can be made in bulk in a conical tube. ii. Dilute each primary antibody needed with the necessary dilution factor (depending on the antibody used) and add 250 μl to the appropriate wells. Place the slide in 4°C overnight. Dilutions can be made in 1.5mL microcentrifuge tubes. iii. If the slide needs be stained on the same day, it can be placed in the 37°C incubator for 2 hours instead of 4°C overnight. iv. Make sure to note on the Immunocytochemistry form the specific antibody placed in the well. d. Secondary Antibody i. Wash the slide 3 times with 500 μl PBS leaving the PBS in the wells 5-7 minutes per rinse. ii. Dilute each secondary antibody (usually 1:1000 to 1:2000, depending on the intensity) needed with PBS and add 250 μl to the appropriate wells. Incubate at 37°C in the incubator for 45 minutes. iii. Wash the slide 5 times with 500 μl PBS leaving the PBS in the wells 5-7 minutes per rinse. iv. The secondary antibody is specific to the primary antibody such as IgG Rabbit or IgG Mouse. The animal it derives from must be the same as the primary antibody. e. Preparing the slide for microscopic examination i. Drop about 5-8 μl of mounting medium (DAPI) to a slide, remove cover slips from the well using tweezers and place them over the slide on the mounting medium gently. ii. Make sure the DAPI covers the entire area of the cover slips. f. Microscopic Examination Examine the slides after 20-30 minutes under fluorescence microscope. Thank you for your interest in ScienCell Products
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3、Question:
Has anyone used these cells to look at myelin production? I work with a virus that may disrupt myelination and I was interested in looking at this in primary tissue culture cells. Thanks! Lee
Answers:
We do not test for myelin production. thank you for your interest.
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