Human Leptin ELISA Kit EKD01019|产品详情|进口试剂采购网

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Human Leptin ELISA Kit

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货号：EKD01019

规格：96T

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Human Leptin ELISA Kit

商品详情参考文献相关资料

Species Reactivity	Homo sapiens (Human)
Format	96 well plate
Detection Range	0-100 ng/ml
Sensitivity	50 ng/ml
Sample Type	Human Serum
Assay Time	2 hours
Application	ELISA
Assay Type	Sandwich
Detection Method	Colorimetric
Shipping Condition	Ambient shipping
Storage Instructions	4°C. Bring all reagents to room temperature before beginning test. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	1 year
Test Principle	The principle of the following enzyme immunoassay test follows a typical two-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for leptin is immobilized onto the microwell plate and another monoclonal antibody specific for a different epitope of leptin is conjugated to biotin. During the first step, leptin present in the samples and standards is bound to the immobilized antibody and to the biotinylated antibody, thus forming a sandwich complex. Excess and unbound biotinylated antibody is removed by a washing step. In the second step, streptavidin-HRP is added, which binds specifically to any bound biotinylated antibody. Again, unbound streptavidin-HRP is removed by a washing step. Next, the enzyme substrate is added (TMB), forming a blue coloured product that is directly proportional to the amount of leptin present. The enzymatic reaction is terminated by the addition of the stopping solution, converting the blue colour to a yellow colour. The absorbance is measured on a microtiter plate reader at 450nm. A set of standards is used to plot a standard curve from which the amount of leptin in patient samples and controls can be directly read.
Assay Precision	The intra assay precision is between a CV% of 3.7-5.0 ng/ml. The inter assay precision is between a CV% of 5.8-6.8 ng/ml.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.