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Human Serum Amyloid A ELISA Kit

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规格：96T

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Human Serum Amyloid A ELISA Kit

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Overview

Name:	Human Serum Amyloid A ELISA Kit See all Serum Amyloid A kits
Applications:	Sandwich ELISA
Assay Type:	Sandwich (quantitative)
Platform:	Microplate
Detection Type:	Colorimetric
Sample Type:	Serum, plasma, cell culture supernatant, and other biological fluids.
Sensitivity:	< 1.1 ng/ml
Range:	1.1-80 ng/ml
Assay Length:	2h 30m
Reactivity:	Human
Cross Reactivity:	This assay has shown no cross-reactivity with other cytokines tested.
Principle of Assay:	This SAA enzyme-linked immunosorbent assay (ELISA) applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for SAA. Standards or samples are then added to the appropriate microtiter plate wells and incubated. SAA if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound SAA and other components of sample. In order to quantitate the amount of SAA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for SAA is added to each well to "sandwich" the SAA immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate solution are allowed to react over a short incubation period. Only those wells that contain SAA and enzyme-substrate reaction will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the colour change measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. In order to measure the concentration of SAA in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus SAA (ng/mL). The concentration of SAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Protein

Function:	Major acute phase protein.
Tissue Specificity:	Expressed by the liver; secreted in plasma (at protein level).
Sequence Similarities:	Belongs to the SAA family.
Post-Translational Modification:	This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
Cellular Location:	Secreted.
Database Links:	Entrez Gene: 6288 Human Entrez Gene: 6289 Human Entrez Gene: 694827 Human Omim: 104750 Human SwissProt: P02735 Human SwissProt: P0DJI8 Human Unigene: 1955 Human Unigene: 632144 Human
Synonyms:	Amyloid fibril protein AA Amyloid fibrils Amyloid protein A Fibrils MGC111216 OC PIG 4 PIG4 SAA SAA 1 SAA 2 SAA1 SAA2 SAA_HUMAN Serum Amyloid A Serum amyloid A protein precursor Serum amyloid A1 isoform 1 Serum amyloid A1 isoform 2 Serum amyloid protein A(4-101) TP53I4 Tumor protein p53 inducible protein 4
Information:	Protein information shown above is from the UniProt Consortium.