Based on N-terminal sequencing, the mature recombinant IL-29 starts at Gly 20 and has a calculated molecular mass of 21.4 kDa. As a result of glycosylation, the recombinant monomer migrates as an approximately 26-35 kDa protein in SDS-PAGE under reducing conditions.

DNA sequence encoding the signal peptide from human CD33 was fused to the carboxyl terminally polyhistidine-tagged mature human IL-29 (Gly 20-Thr 200). The chimeric protein was expressed in a mouse myeloma cell line, NS0.

Greater than 95%
Endotoxin level < 1 EU/μg

Human HepG2 cells infected with encephalomyocarditis virus, ED50 1-5 ng/ml

Phosphate buffered saline (PBS) 

< 1 EU/μg

For retention of full activity store at -70 ºC or below and avoid repeated freeze-thaw cycles