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上海拜力生物科技公司
Tel:400-968-7988    021-33779008
Acc65 I (Kpn I*)
品牌:Vivantis Technologies
货号:FDRE1104
规格:50 preps
货期:

Home Restriction Endonucleases Fast Digest Restriction Endonucleases Acc65 I (Kpn I*) Acc65 I (Kpn I*) Concentration 10u/μl 5 ' . . . G↓G TA C C . . . 3 ' 3 ' . . . C C AT G↑G . . . 5 ' Storage Buffer 10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol

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Home  Restriction Endonucleases  Fast Digest Restriction Endonucleases  Acc65 I (Kpn I*)

Acc65 I (Kpn I*)

Concentration
10u/μl 
5 ' . . . G↓G TA C C . . . 3 '
3 ' . . . C C AT G↑G . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol

Unit Definition
10u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation
65˚C for 20 minutes.

Quality Control Assays
Ligation/ Recutting Assay

After 10-fold overdigestion with Acc65 I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Acc65 I for 16 hours at 37˚C.

Ordering Information

Catalog No Description Pack Size
FDRE1104 Acc65 I (Kpn I*) 50 preps

 

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Acc65 I (Kpn I*)

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