Description
The ViPrimePLUS Yersinia enterolitica qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Yersinia enterolitica genomes in food, milk and water samples.
Yersinia enterolitica belongs to a family of rod-shaped bacteria termed Enterobacteriaceae that consist of 11 species, three of which are pathogenic to humans: Yersinia enterocolitica, Yersinia pestis and the enteropathogenic yersiniae and Yersinia pseudotuberculosis. Y. enterolitica is a psychotropic bacterium causing acute gastro-enteritis and occasionally more serious disease in humans. Acute infections of the bacterium usually lead to mild self-limiting entero-colitis or terminal ileitis in humans. Symptoms may include fever and watery or bloody diarrhoea.
ViPrimePLUS Yersinia enterolitica qPCR Kit is intended for research use only.
Kit Component
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Y. enterolitica Primers and Probe Mix (Y. enterolitica PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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• Food samples
• Water samples
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Gene Target
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Attachment invasion locus protein (ail) gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM2069
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ViPrimePLUS Yersinia enterolitica qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Yersinia enterolitica qPCR Kit
