Concentration
2-5u/μl 
5'…GGTCTC(N)₁^↓…3' 
3'…CCAGG(N)_5↑…5'

Reaction Conditions 
1X Buffer V3 
50mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂, 100mM NaCl, and 100μg/ml BSA. Incubate at 55°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 3-fold overdigestion with Bso31I, 90% of the DNA fragments can be ligated and 80% of the DNA fragments can be recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 2u of Bso31I for 16 hours at 55°C.
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
Bso31I {Eco31I}