Concentration
5-20u/μ
5'…ATGCA↓T…3'
3'…T↑ACGTA…5'

Reaction Conditions 
1X Buffer V1 
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂ and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 20-fold overdigestion with Zsp2I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Zsp2I for 16 hours at 37°C. 
Supplied with 10X Buffer V1, 10X Buffer UB and Viva Buffer A. (Diluent)
Enzyme is stable for up to 6 months if properly stored. It is recommended that the enzyme is kept in small aliquot to avoid repeated freeze-thaw cycles.

Ordering Information

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Manual
Zsp2I {AvaIII}