Concentration
10-30u/μl
5'…G↓CGC…3'
3'…CGC↑G…5'

Reaction Conditions 
0.5X Buffer UB
12.5mM Tris-acetate (pH 7.6 at 30°C), 5mM Mg-acetate, 50mM K-acetate, 3.5mM 2-mercaptoethanol, and 25μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCI, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 10-fold overdigestion with HspAI, more than 90% of the DNA fragments can be ligated recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of HspAI for 16 hours at 37°C. 
Supplied with 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
HspA I {HhaI*}