Concentration
10-40u/μl
5'…C↓TCGAG…3'
3'…GAGCT↑C…5'

Reaction Conditions 
1X Buffer V1
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂, and 100μg/ml BSA. Incubate at 50°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 20-fold overdigestion with Sfr274 I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of Sfr274I for 16 hours at 50°C.
Supplied with 10X Buffer V1, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
Sfr274I