Concentration
10-30u/μl
5'…GT↓AC…3'
3'…CA↑TG…5'

Reaction Conditions 
1X Buffer V1
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl2, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
20mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 20-fold overdigestion with RsaI, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of RsaI for 16 hours at 37°C.
Supplied with 10X Buffer V1, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Downloads
Manual
RsaI

Publication
This Product Has Been Used In: 
Sabet, S., El-Sayed, S.K., Mohamed, H.T., El-Shinawi, M., Mohamed, M.M. (2017). Inflammatory breast cancer: High incidence of GCC haplotypes (−1082A/G, −819T/C, and −592A/C) in the interleukin-10 gene promoter correlates with over-expression of interleukin-10 in patients’ carcinoma tissues, Vol. 39, No. 7 (2017).
Badouei, M.A., Jajarmi, M., Mirsalehian, A., (2015) Virulence profiling and genetic relatedness of Shigatoxin-producing Escherichia coli isolated from humans and ruminants. Comparative Immunology, Microbiology and Infectious Diseases. 38. Pp15-20.
Tappeh, K.H., et al. (2014) Incidence of Giardia lamblia Subspecies by PCR-RFLP in Stool Specimens of Hospitalized Children at Urmia Mutahhari Hospital, West Azerbaijan Province, Iran. Iran Journal of Parasitology. 9(4), p.541-547.