Concentration
2-10u/μl 
5'…CMG↓CKG…3'
3'…GKC↑GMC…5'

Reaction Conditions 
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
20mM Tris-HCl (pH 7.6), 300mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 10mM MgCl₂ 200μg/ml BSA and 50% glycerol. Store at -20°C. Store at -70°C for period longer than 30 days.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 5-fold overdigestion with MspA1I, 60% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of MspA1I for 16 hours at 37°C. 
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
MspA1I {NspBII}