Description
The 2X Ampli-Optimization Kit is designed to provide an easy and convenient way to optimize reaction conditions specific for your amplicon. This kit contains the 2X Taq Master Mix with a range of MgCl₂ concentrations (2.0-7.0mM). Other reaction components included in 2X Taq Master Mix such as dNTPs, reaction buffer and Taq DNA Polymerase are provided at the optimized concentration. This kit enables the user to determine the specific MgCl₂ concentration for their amplification reaction.

Features

• Easy and convenient for optimization of MgCl₂ concentration.
• Saves time and reduces contamination due to reduced number of pipetting steps.
• Color caps are provided for convenient recognition of MgCl₂ concentration within the 2X Taq Master Mix.
• Stable at 4°C for 6 months, allowing immediate reaction setup without the time-consuming thawing of reagents.
• Reaction buffer, dNTPs and Taq DNA Polymerase are provided at an optimized concentration
• Suitable for all routine DNA amplification applications.

Composition 
Taq DNA Polymerase (0.05u /µl), 2X ViBuffer A (100mM KCl, 20mM Tris-HCl (pH9.1 at 20°C) and 0.02% Triton™ X-100), 0.4mM dNTPs and 2.0-7.0 mM MgCl₂ .

Quality Control 
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage & Stability

• Stable at -20°C for one year or at 4°C for 6 months if properly stored.
• Stable for 20 freeze-thaw cycles. To avoid frequent freeze-thaw, keeping small aliquots at -20°C is recommended
• For daily use, keeping aliquots at 4°C is recommended.

Ordering Information

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Manual

2X Ampli-Optimization Kit