Description
Viva 2-step RT-PCR kit is specially designed to provide reliable synthesis of full-length cDNA and convenient application of cDNA in PCR. M-MuLV Reverse Transcriptase synthesize complementary DNA strand initiating from a specific primer, oligo d(T) or random hexamer. The absence of RNase H enhances the synthesis of long cDNA as RNA strand does not degraded in DNA-RNA hybrid during first strand cDNA synthesis. With variety of kit options for standard PCR and long PCR, Viva 2-step RT-PCR kit provides flexibility in an easy use format.

Features

• Absence of RNase H activity allows high amount of full length cDNA synthesis with RNA templates up to 10kb.
• Wide selection of primers, oligo d(T) or random hexamer. Taq DNA Polymerase and MaxTaq DNA Polymerase for amplification for short and long DNA fragments.

Kit Component

Viva cDNA Synthesis Kit

• 10X M-MuLV RT Buffer, 250µl
• 10,000u M-MuLV Reverse Transcriptase
• 10mM dNTPs mix, 0.25ml
• RNase-free water 3ml
• 40µM Oligo d( T ), 100µl
• 50ng/µl Random hexamer, 100µl

Viva PCR Kit

• 500u Taq DNA Polymerase / 500u Chromo Taq DNA Polymerase / 500u MaxTaq DNA Polymerase / 500u Chromo MaxTaq DNA Polymerase
• 10X PCR Reaction Buffers
• 50mM MgCl₂
• 10mM dNTPs Mix, 0.25ml

Storage

• Store at -20°C

Ordering Information

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Manual

Viva 2-Step RT-PCR kit with M-MuLV RT / Taq DNA Polymerase

Viva 2-Step RT-PCR Kit with M-MuLV RT / Chromo Taq DNA Polymerase

Viva 2-Step RT-PCR Kit with M-MuLV RT / MaxTaq DNA Polymerase

Viva 2-Step RT-PCR Kit with M-MuLV RT / Chromo MaxTaq DNA Polymerase