Description
Chromo Pfu DNA Polymerase is an extremely thermostable proofreading DNA polymerase, suitable for applications requiring high temperatures synthesis of DNA. Pfu DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg2+. It exhibits the 3' to 5' proofreading activity. The enzyme is supplemented with inert color tracer dyes.

Features

• Color tracer dyes for ease of visualization of the addition of polymerase to the reaction.
• Use of 1u in every 25μl final reaction allows direct loading and serves as tracking dyes during gel electrophoresis. The blue and pink color dyes migrate approximately at 4kb and 0.3kb respectively on 1% TAE agarose gel.
• Ultra pure recombinant protein allows amplification up to 8kb
• 5X ViBuffer Pfu provided for both short and long amplification.
• Recommended for use in high-fidelity amplification and cloning of blunt-ended amplification products.

Unit Definition 
1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl₂ , 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10μg activated calf thymus DNA and 100μg/ml BSA in a final volume of 50μl.

Supplied With

• 50mM MgCl₂
• 5X ViBuffer Pfu

Quality Control 
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage Buffer 
50mM Tris-HCl (pH 8.0 at 22°C ), 100mM KCl, 0.1% Tween™ 20, 0.1% Nonidet-P40, 0.1mM EDTA, 1mM DTT, color dyes and 50% glycerol. Store at -20°C.

Amplification of 1.5kb DNA fragment using Chromo Pfu DNA Polymerase with 10X ViBuffer A (Lane A1-A4) and 5X ViBuffer Pfu (Lane B1-B4).

1. PCR product using 2U of Chromo Pfu DNA Polymerase
2. PCR product using 1U of Chromo Pfu DNA Polymerase
3. PCR product using 0.5U of Chromo Pfu DNA Polymerase
4. PCR product using 0.25U of Chromo Pfu DNA Polymerase

Amplification of 1.5kb DNA fragment from Lambda DNA using Chromo Pfu DNA Polymerase with 10X ViBuffer S (Lane A1-A4) and 5X ViBuffer Pfu (Lane B1-B4).

1. PCR product using 2U of Chromo Pfu DNA Polymerase
2. PCR product using 1U of Chromo Pfu DNA Polymerase
3. PCR product using 0.5U of Chromo Pfu DNA Polymerase

Ordering Information

Download
Manual

Chromo Pfu DNA Polymerase

Comparison Test Report

Chromo Pfu DNA Polymerase