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ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit
品牌:Vivantis Technologies
货号:QV4005
规格:150 reactions
货期:

Home ViPrimePLUS Real-Time PCR Kits Veterinary Infection qPCR/RT-qPCR Kits Piscean (fish) ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit Description The ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Spring Viremia of Carp Virus genome in fish samples (e.g. liver

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ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit

Description
The ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Spring Viremia of Carp Virus genome in fish samples (e.g. liver, kidney, gills, spleen and brain).
Spring Viremia of Carp Virus (SVCV), a bullet-shaped RNA virus, is responsible for the acute haemorrhagic and contagious viremia in several carp species, and some other cyprinid and ictalurid fish species. The virus has first been identified in Yugoslavia and now, most of the European countries, Russia, the Middle East and even China have been reported of the disease. The virus can be transmitted via the water contaminated with the faeces and urine of the infected carp or via invertebrate vectors. Once the virus enters the host via the gill, the virus spreads rapidly to the liver, kidney, spleen and digestive tract. Diseased fish is usually darker in colour with typical signs of exophthalmia, pale gills, haemorrhages on skin and base of fins, abdominal distension and a protruding anus. Disease usually manifests in water with temperature between 11 and 17oC. Therefore in carp, the disease is often observed during spring period. In European aquaculture, the mortality rate can reach up to 70% in yearling. Vaccine is currently not available, thus effective screening method is essential.
ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit is intended for research use only.

Kit Component

  • SVCV Primers and Probe Mix (SVCV PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit RNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material Fish samples (e.g. liver, kidney, gills, spleen, brain)
Gene Target L2 inner capsid protein VP2
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

Ordering Information

Catalog No Description Pack Size
QV4005 ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit 150 reactions

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ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit

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