Description
The GF-1 Blood DNA Extraction Kit is designed for rapid and efficient purification of genomic DNA from fresh and frozen anti-coagulated whole blood. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA.

Features

• Yields up to 20μg of DNA
• No organic-based extraction required
• Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern blotting and DNA fingerprinting.

Kit Components

• Buffer BB
• Wash Buffer 1 (concentrate)
• Wash Buffer 2 (concentrate)
• Elution Buffer
• Proteinase K

Ordering Information

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Manual

GF-1 Blood DNA Extraction Kit

Stability Test Report

GF-1 Blood DNA Extraction Kit

Publication
This Product Has Been Used In: 
Cilingir, O, Ozkan S., Aras, B.D., Erzurumluoglu, E., Kutlay, O., Akinci, M., Emir, B., Afagh, A., Artan, S., (2017) Association of functional RAGE gene polymorphisms with Parkinson’s disease in a Turkish cohort.Biomedical Research 2017; 28 (19): 8454-8460
Piratae, S., Sae-chue, B., Sukumolanan, P., Phosri, A. (2017). ). Molecular detection of blood pathogens and their impacts on levels of packed cell volume in stray dogs from Thailand, . , Asian Pacific Journal of Tropical Disease , Vol. 7, No. 4, 233-236 (2017).. 
Rani, A., Nawaz, S.K., Irfan, S., Arshad, M., Bashir, R., Shaheen, N. (2017). Role of MyD88-adaptor-like gene polymorphism rs8177374 in modulation of malaria severity in the Pakistani population, The Brazilian Journal of Infectious Diseases, Vol. 21, No. 4. (2017). 
Abajy, M.Y., Ibrahim, A., Almohsen, J.A.(2016). Development of New AS-PCR based Analytical Approach for detecting the Single Nucleotide Polymorphism of AGTR.1 gene, International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, No. 7 (2016).
Babker,A.M.A.A., Gameel, F.E.M.H. (2016). Methylenetetrahydrofolate Reductase C677T Polymorphism in Sudanese Women with Recurrent Spontaneous Abortions, Kuwait Medical Journal, Vol. 48, No. 2, 100-104 (2016).
Zahri, M.K., Emilia, A., Rawi, R.I.M., Taib W.R.W., Sani, A.I., Baig, A.A. (2016) Contribution of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor Ɣ2 gene in relation to obesity. Meta Gene. 10. Pp..39-44. 
Nawaz, S.K., et al. (2015) Role of S180L Polymorphism in Etiology of Malaria Caused by Plasmodium falciparum in a Small Group of Pakistani Population. Bosnian Journal of Basic Medical Sciences. 15(4), p.20-23. 
Nawaz, S.K., Rani, A., Yousaf, M., Noreen, A., Arshad, M. 2015. Genetic etiology of coronary artery disease considering NOS 3 genevariant rs1799983. Vascular. 23(3) pp.270-276 
Naila, R., et al (2013)Elevated genetic deletion of GSTT1 in Pakistani population JÖKULL Journal63(12). 
Skalar, C., Gurbuz, E., Kalay, N., Kaya, M.G. (2013). Higher frequency of 4977574 (the G Allele) on Chromosome 9p21.3 in Patients with Myocardial Infarction as Revealed by PCR-RFLP Analysis (2013) The Tohoku Journal of Experimental Medicine, Vol 230, No. 3, 171-176 (2013).
Amer, H.M. et al. (2011) An Optimized Polymerase Chain Reaction Assay to Identify Avian Virus Vaccine Contamination with Chicken anemia Virus Journal of Veterinary Diagnostic Investigation. Sage Journals. 23, p. 34-40.