Description
The GF-1 Plasmid DNA Extraction Kit is designed for rapid and efficient purification of high copy and low copy plasmid DNA from bacterial lysates. The kit uses the alkaline lysis-SDS method to lyse cells and release plasmid DNA. Special buffers provided in the kit are optimized to enhance binding DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure plasmid DNA.

Features

• Yields up to 20μg of DNA
• Multiple samples can be processed rapidly in less than 30 minutes
• No organic-based extraction required
• Highly pure plasmid DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, ligation, DNA sequencing, transformation, etc.

Kit Components

• Solution 1
• Solution 2
• Buffer NB
• Wash Buffer (concentrate)
• Elution Buffer
• RNase A

Ordering Information

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Manual

GF-1 Plasmid DNA Extraction Kit

Publication
This Product Has Been Used In: 
Dalimi, A., Nasiri, V. (2016). Immunization with KMP11-NTGP96-GFP Fusion of Leishmania major Induced Th1 Platform Immune Response in Susceptible BALB/c mice, Jundishapur Journal of Microbiology, Vol. 9, No. 12 (2016)
Tohidi, F., et al. (2016) Development of a Novel In Vitro Assay for the Evaluation of Integron DNA Integrase Activity.. Biotechnology & Biotechnological Equipment11(57), Taylor and Francis Online, p.1-7. 
Rouhizadeh, A., Ghadiri, A.A., Jalali, M.R., Ghorbanpour, M., Jalali, M.H.R. Cloning and Sequencing of Truncated Toxoplasma gondii Subtilisin-Like 1 Antigen (2016), Journal of Research in Medical Sciences, Vol. 18, No. 8 (2016).
Uttatree,S., Charoenpanich, J. (2016) Isolation and characterization of a broad pH- and temperature-active, solvent and surfactant stable protease from a new strain of Bacillus subtilis. . Biocatalysis and Agricultural Biotechnology. 8. Pp.32-38 
Ahmadi, F., Sajedi, R.H., Mahdavi, A., Zeinoddini, M., Taghdir, M. (2015). Directed Improvement of i-Photina Bioluminescence Properties, an Efficient Calcium-Regulated Photoprotein, Biomacromolecular Journal, Vol. 1, No. 1, 80-92 (2015).
Beige, F., Salehi, M.B., Bahador, N., Mobasherzadeh, S. (2015). Plasmid Mediated Antibiotic Resistance in Isolated Bacteria From Burned Patients Jundishapur Journal of Microbiology,Vol. 8, No. 1, (2015).
Hamidinejat, H., et al. (2015) Development of an Indirect ELIS using Different Fragments of Recombinant Ncgra 7 for Detection of Neospora caninum Infection in Cattle and Water Buffalo. BIran Journal of Parasitology. 10(1), p.69-77. 
Hesampour, A., et al. (2014) Comparison of Biochemical Properties of Recombinant Phytase Expression in the Favorable Methylotrophic Platforms of Pichia pastoris and Hansenula polymorpha. Progress in Biological Sciences. 4(1), p. 97 – 111. 
Gul, R., et al. (2012) Expression and Sequence Characterization of Growth Hormone Binding Protein of Nili-Ravi Buffaloes (Bubalus bubalis). African Journal of Biotechnology. ProQuest. 11(57), p. 12103-12109 
Gul, R., et al. (2012) Expression and Sequence Characterization of Growth Hormone Binding Protein of Nili-Ravi Buffaloes (Bubalus bubalis). African Journal of Biotechnology. ProQuest. 11(57), p. 12103-12109 
Pongsilp, N., et al (2012) Genotypic Diversity among Rhizospheric Bacteria of Three Legumes Assessed by Cultivation-dependent and Cultivation-independent Techniques. World Journal of Microbiology and Biotechnology.ProQuest. 28, p. 615-626 
Charoenpanich, J., Suktanarag, S., & Toobbucha, N. (2011)Production of Thermostable Lipase by Aeromonas sp. EBB-1 Isolated from Marine Sludge in Angsila, Thailand ScienceAsia37: 105-114. 
Uttatree, S., Winayanuwattikun, P., & Charoenpanich, J. (2010) Isolation and Characterization of a Novel Thermophilic-organic Solvent Stable Lipase from Acinetobacter baylyi Applied Biochemistry and Biotechnology.