Description
The GF-1 Tissue DNA Extraction Kit is designed for rapid and efficient purification of genomic DNA from various tissue samples such as kidney, heart, lungs, brain, muscles, liver, spleen, and animal cultured cells. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA.

Features

• Yields up to 20μg of DNA
• No organic-based extraction required.
• Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern blotting and DNA fingerprinting.

Kit Components

• Buffer TL
• Lysis Enhancer
• Buffer TB
• Wash Buffer (concentrate)
• Elution Buffer
• Proteinase K

Ordering Information

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Manual

GF-1 Tissue DNA Extraction Kit

Stability Test Report

GF-1 Tissue DNA Extraction Kit

Publication
This Product Has Been Used In: 
Malaitad, T., Poeaim, S., Eiamampai, K. (2015). Sex identification in barn swallows (Hirundorustica Linnaeus) by molecular technique, Journal of Agricultural Technology, Vol. 11, No. 8, 2411-2418 (2015). 
Abdossamadi, Z., Seyed, N., Zahedifard, F., Taheri, T., Taslimi, Y. (2017) )Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice. . PLOS Neglected Diseases Vol. 11, No. 12 (2017).
Ghatar, R.H., Soltantoyeh, T., Bahadori, T., Golara. M., Hassania, H., Eghbal, R.K., Khoshnoodi, J., Judaki, M.A., Golsaz-Shirazi, F., Jeddi-Tehrani, M., Amiri, M.M., Shokri, F., (2017) Epitope Mapping of Human HER2 Specific Mouse Monoclonal Antibodies Using Recombinant Extracellular Subdomains. Asian Pacific Journal of Cancer Prevention Vol 18 DOI:10.22034/APJCP.2017.18.11. 31033
Kim, Y.N., Dickinson, N., Bowie, M., Robinson, B., Boyer, S. (2017). Molecular identification and distribution of native and exotic earthworms in New Zealand human-modified soils, New Zealand Journal of Ecology, Vol 41, No. 23 (2017).
Alabsi, A.M., et al. (2016) Cell Cycle Arrest and Apoptosis Induction via Modulation Mitochondrial Integrity by Bcl-2 Family Members and Caspace Dependence in Dracaena cinnabari-Treated H400 Human Oral Squamous Cell Carcinoma. BioMed Research International. 
Chontananarth, T. (2016) Multiplex PCR assay for discrimination of Centrocestus caninus and Stellantchasmus falcatus. Asian Pacific Journal of Tropical Biomedicine. 
Chuah,L.O., He, X.B., Effarizah, M.E., Syahariza, Z.A., Shamila-Syuhada, A.K., Rusul, G. (2016) Mislabelling of beef and poultry products sold in Malaysia. Food Control. 62. Pp157-164 
Abdo el Motalab, Y.S., & Ahmed, A.B. (2014) Isolation and Identification of Camelpox Virus in Eastern Sudan SUST Journal of Agricultural and Veterinary Sciences (SJAYS). 15(2), p. 73-81. 
Chontananarth, T., Wongsawad, C., Chomdej, S., Krailas, D., Chai, J.Y. (2014) Molecular phylogeny of trematodes In Family Heterophyidae based on mitochondrial cytochrome c oxidase subunit I (mCO I). Asian Pacific Journal of Tropical Medicine. Pp..446-450. 
Noikong, W., Wongsawad, C. (2014) ) Epidemiology and molecular genotyping of echinostome metacercariae in Filopaludina snails in Lamphun Province, Thailand. Asian Pacific journal of tropical Medicine. 7(1). Pp.26-29.
Ayaz, S., Islam, N.M.A.E., Rubab, L., Ullah, R., Khan, S., Hussain, R. (2013). Prevalence and Molecular Detection of Giardia and Cryptosporidium spp in Communities Consuming Different Drinking Water Sources in Kohat, Khyber Pakhtunkhwa Pakistan, Life Science Journal, Vol. 10, No. 1 (2013).
Sade, A., & Biun, H. (2009)Detection of Pig Residue in Fish Feedstuffs and Fish Stomach Content Using Polymerase Chain Reaction for Halal Certification Proceedings of the 5th National Fisheries Symposium 2008: Kuala Terengganu (Malaysia), 2008: 143-148