Description
Avian Myeloblastosis Virus (AMV) Reverse Transcriptase is an RNA-dependent DNA polymerase (aß holoenzyme) with molecular weight of 157 kDa. It synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template.

Concentration
20u/μl

Assay Condition 
50mM Tris- HCl (pH8.3), 6mM MgCl₂, 40mM KCl, 4mM DTT, 0.5mM [ ³H]-TTP (10-20c/m/pmol), 0.4mM Poly(rA)•(dT)_12-18, in a reaction volume of 25μl.

Supplied With 
10X Buffer AMV-RT 
250mM Tris-HCl (pH 8.3), 500mM KCl, 50mM MgCl₂ and 20mM DTT. Store at -70˚C.

Storage Buffer
200mM Potassium Phosphate (pH 7.2), 0.2% TritonTM X-100, 2mM DTT and 50% glycerol. Store at -70˚C for long term. Store at -20˚C for short term (1 month). .

Thermal Inactivation 
80˚C for 10 minutes.

Unit Definition 
1u is defined as the amount of enzyme that is required to incorporate 1nmol of dMTP into an acid-insoluble material in 10 minutes at 37˚C using Poly(rA)•(dT)_12-18 as a template primer.

Features

• First strand synthesis of cDNA.
• Synthesis of cDNA for cloning.
• cDNA labelling.
• Primer extensions and RNA sequencing
• RT-PCR
• Dideoxy sequencing of DNA and RNA.

Quality Control 
All preparations are assayed for contaminating endonuclease, exonuclease and non-specific RNase activities.

Ordering Information

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Manual

AMV Reverse Transcriptase (Recombinant)

Publication
This Product Has Been Used In:
Lim et al. (2012) Co-administration of Avian Influenza Virus H5 Plasmid DNA with Chicken IL-15 and IL-18 Enhanced Chickens Immune Responses. BMC Vet Res8: 132.