Description
Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA dependent DNA polymerase. It can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template. The absence of RNase H activities enhances the synthesis of long cDNAs and therefore the enzyme is recommended for preparing long cDNAs.

Concentrations 
100 - 500u/μl

Assay Conditions 
50mM Tris- HCl (pH8.3), 6mM MgCl₂, 10mM DTT, 0.4mM Poly(rA)•(dT)12-18, and 0.5mM [ ³H]dTTP in a reaction volume of 50μl./μl

Supplied With 
10X Buffer M-MuLV RT 
500mM Tris-HCl (pH 8.3 at 25˚C), 75mM KCl, 3mM MgCl₂ and 10mM DTT. Store at -20˚C.

Storage Buffer 
10mM K-Phosphate (pH 7.5), 0.1mM EDTA, 200mM NaCl, 7mM 2-mercaptoethanol and 50% glycerol. Store at -20˚C.

Thermal Inactivation 
70˚C for 10 minutes

Unit Definition 
1u is defined as the amount of enzyme that is required to incorporate 1nmol of dTTP into an acid-insoluble material in 10 minutes at 37˚C using Poly(rA)•oligo(dT).

Application

• First strand cDNA synthesis.
• DNA labeling.
• RNA analysis by primer extension.

Quality Control
Purified free of contaminating endonucleases and exonucleases.

Ordering Information

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Manual

M-MuLV Reverse Transcriptase (RNase H¯)

Publication
This Product Has Been Used In:
Kaplan, S. et al. (2016) The Potential of Microarray Databases to Identify Tissue Specific Genes Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(1), p. 29-35. 
Noureini, S.K., Tanavar, F. (2015) Boldine, a natural aporphine alkaloid, inhibits telomerase at non-toxic concentrations. Chemico-Biological Interactions. 231. Pp27-34. 
Tahoori, M.T., Pourfathollah, A.A., Soleimani, M., Vasheghani-Farahani, E., Mohammadzadeh, A., Amari, A., Hashemi, S.M., mossahebi-mohammadi, M. (2015) Fibroblasts feeder niche and Flt3 Ligand as a novel inducer of plasmacytoid dendritic cells development in vitro. International Immunopharmacology. 24. Pp.474-480.
Ahmadi, E., Ahmadi, M., PourBahsh, S.A., Talebi, A. (2013). Detection and Differentiation of Newcastle Disease Virus Strains Affecting Commercial Poultry in Northwest of Iran using RT-PCR,International Journal of Veterinary Science, Vol 2, No. 4, 138-142. 
Supathaweewat, K., Klanrit, P (2013). cDNA cloning and expression analyses of phytoene synthase 1, phytoene desaturase and -carotene desaturase genes from Solanum lycopersicum KKU-T34003, Songklankarin Journal of Science and Technology, Vol. 35, No. 5, 517-527 (2013).