Description
The ViPrimePLUS Bovine Herpesvirus 1 qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Bovine Herpesvirus 1 genome in tracheal swab, genital fluids and semen.
Bovine herpesvirus 1 (BHV-1), a double-stranded DNA virus, is an important cattle pathogen that contributes to the major economic losses in the industry. It is one of the causing agents for the Bovine Respiratory Disease, a severe pneumonia disease. The infection also causes venereal diseases, encephalitis, conjunctivitis, abortion, and systemic infection in neonate calves. There are several virus subtypes, such as BHV-1.1 which is known as the respiratory form, BHV-1.2 as the genital form and BHV-1.3 as the encephalitic form. However, the distinction is not exclusive as there are cases of respiratory infection by BHV-1.2 and intrauterine infection by BHV-1.1. Latent infection is observed in the nervous system, where the viral reactivation promotes virus transmission and recurrent disease occurrence. Many commercially available vaccines using modified live-attenuated virus cause illnesses in young calves due to the immunosuppression property of the virus.
ViPrimePLUS Bovine Herpesvirus 1 qPCR Kit is intended for research use only.
Kit Component
-
BHV-1 Primers and Probe Mix (BHV-1 PPM)
-
Positive Control
-
Internal Extraction Control Primers and Probe Mix (IEC PPM)
-
Internal Extraction Control (IEC DNA)
-
Nuclease Free Water
-
Template Preparation Buffer
Product Specification
Technology
|
Taqman probe-based real-time PCR assay
|
Type of Nucleic Acid Kit
|
DNA
|
Kit Storage
|
Shipped in room temperature, store at -20℃ on arrival
|
Sample Material
|
Ruminant samples (e.g. tracheal swab, genital fluids, semen)
|
Gene Target
|
Putative fibronectin binding protein
|
Detection Limit
|
10 to <100 copies per reaction
|
Sensitivity & Specificity
|
Ct value 28±3 with probability of 95%
|
Controls Included
|
• Internal Extraction Control (IEC) as inhibition control
• Positive control
|
Channels
|
• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
|
Ordering Information
Catalog No
|
Description
|
Pack Size
|
QV6008
|
ViPrimePLUS Bovine Herpesvirus 1 qPCR Kit
|
150 reactions
|
Download
Manual
ViPrimePLUS Bovine Herpesvirus 1 qPCR Kit