Baboon, Dog, Horse, Rabbit, Guinea Pig, Rat, Mouse, Cat, Hamster, Pig, Rhesus macaque

2°C to 8°C  (≤ 30 days)

For long-term storage (> 30 days), aliquot and store at ≤ –20°C. Avoid repeated freeze-thaw.

For Research Use Only. Not for use in diagnostic procedures

Highly purified human C5 was isolated from normal serum and used to immunize goats. The anti-human C5 polyclonal antisera was tested against normal human plasma by double immunodiffusion, one-dimensional immunoelectrophoresis, quantitative radial immunodiffusion, and quantitative rocket immunoelectrophoresis. The antiserum was determined to be monospecific for C5 at varying concentrations.

C5, is a plasma glycoprotein which is present in normal human serum/plasma at approximately 70 µg/mL, and is key to all complement pathways. C5 is synthesized by a wide variety of cells, including liver hepatocytes, epithelial cells of the genitourinary and intestinal tract, and phagocytic mononuclear cells. The precursor protein to C5 (pro-C5) is a single chain polypeptide which undergoes proteolytic cleavage to yield the circulating form of C5. This circulating form contains 2 disulfide-bonded subunits: α – 115 kD, β – 75 kD, which gives the intact C5 protein an approximate total molecular weight of 190 kD. Activation of the classical or alternative complement pathway results in the assembly of C5 convertase enzymes (C4b,2a,3b and C3b,Bb,C3b) on the target cell surface. Both convertase enzymes cleave C5 into C5a and C5b

Applications of the C5 polyclonal antisera have been evaluated by various research facilities, and include, Western Blot, 1,2 IHC, 3,4 Immunofluorescence, 5 and Flow Cytometry. 6