Product HiGlo Fluorescent Western Blocking Buffer Description Blocking Buffer for Fluorescent Western Blotting. HiGlo Blocking Buffer is specifically designed for western blotting using fluorochrome conjugated antibodies. Product Group Accessory reagents Buffer Proprietary protein formulation in TRIS buffered saline at pH 7.6 Preservative Thimerosal Sterility 0.22 µm filtration Appearance