Cyanine3 NHS ester是标记生物分子的氨基基团的活性染料,是Cy3® NHS ester的类似物。该荧光染料是可溶性蛋白、多肽和寡核苷酸/DNA的理想标记物。对于痕量蛋白质,可以考虑用水溶的不需要共溶剂的sulfo-Cyanine3 NHS ester。 Cyanine3 NHS ester可以替代Cy3®, Alexa Fluor 546, and DyLight 549。
Cy® is a trademark of GE Healthcare.
General properties
Appearance:
red powder
Mass spec M+ increment:
474.2
Molecular weight:
590.15
Molecular formula:
C34H40ClN3O4
Solubility:
soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control:
NMR 1H and HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:
Download
Spectral properties
Excitation maximum, nm:
555
Extinction coefficient at excitation maximum, Lmol-1cm-1:
150000
Emission maximum, nm:
570
Fluorescence quantum yield:
0.31
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