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Cyanine5 NHS ester
品牌:
货号:13020
规格:
货期:

订购货号 产品名称及规格 价格(¥) 13020 Cyanine5 NHS ester, 1 mg 1430.00 23020 Cyanine5 NHS ester, 5 mg 2730.00 43020 Cyanine5 NHS ester, 25 mg 5330.00 53020 Cyanine5 NHS ester, 50 mg 9035.00 63020 Cyanine5 NHS ester, 100 mg 15470.00

商品详情 参考文献 相关资料

Cyanine5 NHS ester

Cyanine5 NHS ester

详细描述

 

订购货号 产品名称及规格 价格(¥)
13020 Cyanine5 NHS ester, 1 mg 1430.00
23020 Cyanine5 NHS ester, 5 mg 2730.00
43020 Cyanine5 NHS ester, 25 mg 5330.00
53020 Cyanine5 NHS ester, 50 mg 9035.00
63020 Cyanine5 NHS ester, 100 mg 15470.00

 

 


在过去的几年里,CY5荧光团已经成为生命科学研究和诊断领域非常流行的分子标记物。这些荧光素的*强发射光为红光,在这个范围大多数CCD检测器具有*大检测灵敏度,而且是生物物质低背景区。染料的颜色是非常强烈的,因此在凝胶电泳中低至1nmol的量都能被肉眼观测到。CY5 NHS ester是用于标记多肽、蛋白和寡核苷酸的氨基基团的活性染料。在标记反应中该染料需要少量的有机共溶剂(如DMF,DMSO)(请参照我们的推荐手册以获得更多帮助信息)。对于可溶性蛋白、各种各样的多肽和寡核苷酸而言,这种染料是非常理想而且成本低廉的。该染料在有机溶剂对小分子物质的标记也是非常有效的。对于更多的精细目标物,如易降解蛋白,如果DMF或DMSO会对其有影响,可以考虑water-soluble Cy5 NHS ester(水溶性Cy5 NHS ester染料),因为它们不需要有机溶剂助溶,而且具有和荧光染料类似的性质。

 

CY5荧光团可以与很多的仪器如荧光显微镜、成像仪、扫描仪、荧光分析仪等相兼容。许多的CY5衍生物——CY5 NHS ester可以替代Alexa Fluor 647和 DyLight 649等活化酯(activated esters)。

 

 

图片关键词

 

推荐手册

氨基分子的NHS酯标记

 


Cy® is a trademark of GE Healthcare.


General properties

 

Appearance:

dark blue powder

Molecular weight:

616.19

Molecular formula:

C36H42ClN3O4

CAS number:

1032678-42-4, 350686-88-3

Solubility:

good in polar (DMSO, DMF) and chlorinated (DCM, chlroform) organic solvents, low solubility in water

Quality control:

NMR 1H (95%) and 13C, TLC, functional testing

Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

MSDS:

Download

Spectral properties

 

Excitation maximum, nm:

646

Extinction coefficient at excitation maximum, Lmol-1cm-1:

250000

Emission maximum, nm:

662

Fluorescence quantum yield:

0.2

 


Product citations

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  • Hu, X.; Wang, Q.; Liu, Y.; Liu, H.; Qin, C.; Cheng, K.; Robinson, W.; Gray, B.D.; Pak, K.Y.; Yu, A. et al.Optical imaging of articular cartilage degeneration using near-infrared dipicolylamine probes.Biomaterials2014, 35(26), 7511-7521. doi: 10.1016/j.biomaterials.2014.05.042
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  • Zhang, Y.; Ge, C.; Zhu, C.; Salaita, K. DNA-based digital tension probes reveal integrin forces during early cell adhesion. Nature Communications2014, 5, 5167-5167. doi: 10.1038/ncomms6167
  • Chen, H.; Xiao, L.; Anraku, Y.; Mi, P.; Liu, X.; Cabral, H.; Inoue, A.; Nomoto, T.; Kishimura, A.; Nishiyama, N. et al. Polyion Complex Vesicles for Photoinduced Intracellular Delivery of Amphiphilic Photosensitizer. Journal of the American Chemical Society2014, 136(1), 157-163. doi:10.1021/ja406992w
  • Albertazzi, L.; Martinez-Veracoechea, F.J.; Leenders, C.M.A.; Voets, I.K.; Frenkel, D.; Meijer, E.W.Spatiotemporal control and superselectivity in supramolecular polymers using multivalency.Proceedings of the National Academy of Sciences2013, 110(30), 12203-12208. doi:10.1073/pnas.1303109110
  • Cheng, M.-C.; Leske, A.T.; Matsuoka, T.; Kim, B.C.; Lee, J.; Burns, M.A.; Takayama, S.; Biteen, J.S. Super-Resolution Imaging of PDMS Nanochannels by Single-Molecule Micelle-Assisted Blink Microscopy.The Journal of Physical Chemistry B2013, 117(16), 4406-4411. doi: 10.1021/jp307635v
  • Haller, A.; Altman, R.B.; Souliere, M.F.; Blanchard, S.C.; Micura, R. Folding and ligand recognition of the TPP riboswitch aptamer at single-molecule resolution. Proceedings of the National Academy of Sciences2013, 110(11), 4188-4193. doi: 10.1073/pnas.1218062110
  • He, H.; Chen, S.; Zhou, J.; Dou, Y.; Song, L.; Che, L.; Zhou, X.; Chen, X.; Jia, Y.; Zhang, J. et al.Cyclodextrin-derived pH-responsive nanoparticles for delivery of paclitaxel. Biomaterials2013, 34(21), 5344-5358. doi: 10.1016/j.biomaterials.2013.03.068
  • Rimpelová, S.; B?íza, T.; Králová, J.; Záruba, K.; Kejík, Z.; Císa?ová, I.; Martásek, P.; Ruml, T.; Král, V.Rational Design of Chemical Ligands for Selective Mitochondrial Targeting. Bioconjugate Chemistry,2013, 24(9), 1445-1454. doi: 10.1021/bc400291f
  • Soulière, M.F.; Altman, R.B.; Schwarz, V.; Haller, A.; Blanchard, S.C.; Micura, R. Tuning a riboswitch response through structural extension of a pseudoknot. Proceedings of the National Academy of Sciences2013, 110(35), E3256-E3264. doi: 10.1073/pnas.1304585110
  • Yang, H.; Mao, H.; Wan, Z.; Zhu, A.; Guo, M.; Li, Y.; Li, X.; Wan, J.; Yang, X.; Shuai, X. et al. Micelles assembled with carbocyanine dyes for theranostic near-infrared fluorescent cancer imaging and photothermal therapy. Biomaterials2013, 34(36), 9124-9133. doi: 10.1016/j.biomaterials.2013.08.022
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  1. Chiang, W.-L.; Lin, T.-T.; Sureshbabu, R.; Chia, W.-T.; Hsiao, H.-C.; Liu, H.-Y.; Yang, C.-M.; Sung, H.-W. A rapid drug release system with a NIR light-activated molecular switch for dual-modality photothermal/antibiotic treatments of subcutaneous abscesses. Journal of Controlled Release,2015, 199, 53–62. doi: 10.1016/j.jconrel.2014.12.011
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  3. Bamrungsap, S.; Apiwat, C.; Chantima, W.; Dharakul, T.; Wiriyachai****, N. Rapid and sensitive lateral flow immunoassay for influenza antigen using fluorescently-doped silica nanoparticles.Microchimica Acta2014, 181(1–2), 223-230. doi: 10.1007/s00604-013-1106-4
  4. Duellberg, C.; Trokter, M.; Jha, R.; Sen, I.; Steinmetz, M.O.; Surrey, T. Reconstitution of a hierarchical +TIP interaction network controlling microtubule end tracking of dynein. Nature Cell Biology2014, 16(8), 804-811. doi: 10.1038/ncb2999
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